WSSC Water Quality Monitoring



Here are the 17th round of results of WSSC's fecal bacteria monitoring tests which are required by the consent decree. Many sites had high Enterococci bacteria counts this time. These results are from this WSSC report.

March 29, 2011

The Maryland state standards for Enterococci bacteria:
All Areas: 33
Frequent Full Body Contact Recreation:61
Moderately Frequent Full Body Contact Recreation:78
Occasional Frequent Full Body Contact Recreation:107
Infrequent Full Body Contact Recreation:151
All numbers are counts per 100 milliliters
Stream SiteFecal Bacteria Results
Enterococcus CFU/100mL
Bacterial Source Testing
HumanAvianCanineDeerHorseMiscellaneous WildlifeNon-human Unknown
1). Broad Creek
(downstream - Henson Creek)
<10Too few bacteria for bacteria source determination
1). Broad Creek
(upstream - Henson Creek)
<10Too few bacteria for bacteria source determination
1). Cabin John Creek
(upstream)
<10
Bacteriodes: Negative
Too few bacteria for bacteria source determination
1). Horsepen
(upstream)
<10
(<10)
Bacteroides: Negative
Too few bacteria for bacteria source determination
1). Northeast Branch
(upstream)
<10Too few bacteria for bacteria source determination
1). Paint Branch
(upstream)
<10Too few bacteria for bacteria source determination
1). Piscataway
(upstream)
<10Too few bacteria for bacteria source determination
1). Rock Creek
(upstream)
<10Too few bacteria for bacteria source determination
1). Seneca Creek
(downstream)
<10Too few bacteria for bacteria source determination
1). Upper Beaverdam Creek
(downstream)
<10Too few bacteria for bacteria source determination
1). Upper Beaverdam Creek
(upstream)
<10Too few bacteria for bacteria source determination
1). Watts Branch
(downstream)
<10Too few bacteria for bacteria source determination
1). Watts Branch
(upstream)
<10Too few bacteria for bacteria source determination
1). Western Branch
(downstream)
<10Too few bacteria for bacteria source determination
1). Western Branch
(upstream)
<10Too few bacteria for bacteria source determination
16). Muddy Branch
(upstream)
15
(<10)
Too few bacteria for bacteria source determination
Stream SiteFecal Bacteria Results
Enterococcus CFU/100mL
Bacterial Source Testing
HumanAvianCanineDeerHorseMiscellaneous WildlifeNon-human Unknown
16). Lower Anacostia
(upstream)
150%43%12%10%14%21%
16). Northeast Branch
(downstream)
150%37%12%14%24%13%
19). Little Falls
(upstream)
200%33%9%12%27%19%
19). Northwest Branch
(downstream)
20
Bacteriodes: Negative
0%35%11%16%22%16%
21). Indian Creek
(downstream)
250%35%9%9%17%17%13%
21). Northwest Branch
(upstream)
25
Bacteriodes: Negative
0%39%7%12%27%15%
23). Indian Creek
(upstream)
280%30%11%14%13%23%9%
23). Seneca Creek
(upstream)
280%37%9%13%18%23%
25). Parkway
(upstream - Walker Branch)
30
Bacteriodes: Negative
0%39%13%9%21%18%
26). Paint Branch
(downstream)
350%42%14%15%6%14%9%
27). Sligo Creek
(upstream)
38
Bacteriodes: Negative
0%39%11%9%21%20%
28). Muddy Branch
(downstream)
400%43%10%9%21%17%
29). Little Falls
(downstream)
430%29%11%13%24%23%
30). Lower Anacostia
(downstream)
530%47%8%12%19%14%
31). Piscataway
(downstream)
580%46%10%14%19%11%
32). Rock Creek
(downstream)
600%39%7%15%22%17%
33). Oxon Run
(upstream-Watts Branch)
70
Bacteriodes: Negative
0%46%10%11%27%6%
34). Cabin John Creek
(downstream)
90
(88)
Bacteriodes: Negative
0%
(0%)
41%
(42%)
5%
(5%)
15%
(13%)
23%
(24%)
16%
(16%)
35). Parkway
(downstream - Bear Branch)
93
Bacteriodes: Negative
5%45%12%12%19%7%
36). Lower Beaverdam Creek
(downstream)
148
Bacteriodes: Positive
7%37%12%8%21%15%
37). Oxon Run
(downstream)
243
(315)
Bacteriodes: Negative
(Negative)
0%
(0%)
44%
(41%)
14%
(13%)
9%
(12%)
20%
(19%)
13%
(15%)
38). Horsepen
(downstream)
275
Bacteriodes: Positive
16%32%7%11%12%13%9%
39). Sligo Creek
(downstream)
825
Bacteriodes: Negative
7%33%9%14%19%18%
40). Lower Beaverdam Creek
(upstream)
1025
Bacteriodes: Positive
13%34%17%11%17%8%
Numbers in parentheses() are results for field duplicate samples.

Note from the report:

OTHER MATTERS TO BE BROUGHT TO THE ATTENTION OF EPA, MDE AND THE CITIZENS GROUPS:

As indicated in the text under Article Two, B.5- Water Quality Monitoring (WQM) Plan, under the Polymerase Chain Reaction (PCR) testing protocol for the approved WQM Plan, human source bacteria were identified in one or more samples from four sewer basins. In light of the rapid evolution of Bacterial Source Tracking (BST) technologies, the WQM Plan recommends a periodic re-evaluation of the results of the PCR testing protocol and the methodologies being used. The Virginia Tech laboratory that provides the BST testing services, and which is at the forefront of BST methods development, has recently noted that the current library-based PCR testing protocol may, from time to time, result in false positives where reported human percentage allocations are below 10%. In order to ensure that WSSC receives and presents the most accurate analysis of the presence of human source bacteria, the Virginia Tech laboratory performed additional library-independent human Bacteroides gene testing on a subset of the March 2011 samples. This additional testing revealed false positives in two of the basins sampled, Parkway and Sligo Creek, where the reported percentage allocations were 5% and 7%, respectively. Consequently, application of this additional testing for the Second Quarter of 2011 leads to the conclusion that human source bacteria were identified in one or more samples from only two sewer basins.